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Ensaio redobrando intracelular
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Ensaio redobrando intracelular

Article DOI: 10.3791/3540-v • 07:19 min • January 24th, 2012
January 24th, 2012 •
1, 1
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology

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Neste protocolo um método para medir refolding proteína intracelular após choque térmico é descrito. Este método pode ser usado para estudar foldases como chaperonas moleculares e seus co-fatores ou compostos capazes de influenciar a sua actividade. Firefly luciferase atividade é utilizado como repórter para medir a atividade refolding acompanhante.

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Biologia Molecular acompanhante redobrando estresse choque calor luciferase
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