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Journal / Neuroscience / Funksjonell Kalsium Imaging in Developing…
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Neuroscience
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JoVE Journal Neuroscience
Funksjonell Kalsium Imaging in Developing kortikal Networks
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Funksjonell Kalsium Imaging in Developing kortikal Networks

Article DOI: 10.3791/3550-v • 16:34 min • October 22nd, 2011
October 22nd, 2011 •
1, 1, 1, 1
1Department of Integrative Neurophysiology, VU University, Amsterdam

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Spontan aktivitet for utvikling av nevrale nettverk kan måles ved hjelp av AM-ester former for kalsium-sensitive indikator fargestoffer. Endringer i intracellulær kalsium, noe som indikerer neuronal aktivering, oppdages som forbigående endringer i indikator fluorescens med ett-eller to-foton imaging. Denne protokollen kan tilpasses en rekke developmentally-avhengige nevrale nettverk

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Nevrovitenskap kalsium bildebehandling mus nettverk utvikling cortex multiphoton
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