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JoVE Journal
Biology

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改良酵母双杂交系统,以确定蛋白与生长因子Progranulin的相互作用
 
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改良酵母双杂交系统,以确定蛋白与生长因子Progranulin的相互作用

Article DOI: 10.3791/3562-v 07:57 min January 17th, 2012
January 17th, 2012

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Summary

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我们已经修改了传统的酵母双杂交筛选,确定蛋白质相互作用的一种有效的遗传工具。此修改的过程明显缩短,减少了工作量,最重要的是,降​​低了误报数量。此外,这种方法是重复的,可靠的。

Tags

分子生物学,59期,改良酵母双杂交筛选,PGRN,TNFR2,炎症,自身免疫性疾病
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