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Metaboliseringsvej Bekræftelse og Discovery Gennem 13 C-mærkning af proteinogene aminosyrer
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Metaboliseringsvej Bekræftelse og Discovery Gennem 13 C-mærkning af proteinogene aminosyrer

Article DOI: 10.3791/3583-v • 07:27 min • January 26th, 2012
January 26th, 2012 •
1, 2, 1, 1, 3, 1
1Department of Energy, Environmental and Chemical Engineering, Washington University, 2Department of Biology, Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University

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13 C-isotop mærkning er en teknik til bestemmelse af cellens centrale stofskifte for forskellige typer af mikroorganismer. Efter at cellerne har været dyrket med en bestemt mærket substrat, kan GC-MS måling afslører funktionelle stofskifteveje baseret på unikke mærkning mønstre i proteinogene aminosyrer.

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Molekylær Biologi GC-MS nye vej stofskifte mærkning phototrophic mikroorganisme
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