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Confirmação via metabólica e descoberta através 13 C-rotulagem de proteinogénicos Aminoácidos
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Confirmação via metabólica e descoberta através 13 C-rotulagem de proteinogénicos Aminoácidos

Article DOI: 10.3791/3583-v • 07:27 min • January 26th, 2012
January 26th, 2012 •
1, 2, 1, 1, 3, 1
1Department of Energy, Environmental and Chemical Engineering, Washington University, 2Department of Biology, Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University

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13 C-isótopo rotulagem é uma técnica útil para determinar o metabolismo da célula central para vários tipos de microorganismos. Depois que as células foram cultivadas com um substrato específico rotulados, GC-MS medição pode revelar vias metabólicas funcionais com base em padrões de rotulagem única no proteinogénicos aminoácidos.

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Biologia Molecular GC-MS nova via o metabolismo rotulagem microorganismo fototróficas
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