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Journal / Immunology and Infection / Bestemmelse af fagocyterende Aktivitet of…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bestemmelse af fagocyterende Aktivitet of Clinical Antibody prøver
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Bestemmelse af fagocyterende Aktivitet of Clinical Antibody prøver

Article DOI: 10.3791/3588-v • 09:06 min • November 30th, 2011
November 30th, 2011 •
1, 1, 1, 1, 1, 2
1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

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Vi præsenterer en high-throughput flowcytometrisk analyse for at bestemme fagocyterende aktivitet af antigen-specifikke antistoffer fra kliniske prøver, at udnytte fluorescerende antigen-belagt perler og en monocytic cellelinie udtrykker flere Fc receptorer-leverandører receptor brug og fagocyterende aktivitet bestemmelser på en standardiseret og reproducerbare mode for enhver antigen af ​​interesse.

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Immunologi fagocytose Antibody ADCC Effector Function Fc receptor antistof-afhængige phagocytosis monocytter
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