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Disseksjon og kultur av Chick Statoacoustic ganglion og Spinal Cord explants i Collagen Gels for neurite utvekst Assays
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Disseksjon og kultur av Chick Statoacoustic ganglion og Spinal Cord explants i Collagen Gels for neurite utvekst Assays

Article DOI: 10.3791/3600-v • 11:08 min • December 20th, 2011
December 20th, 2011 •
1, 1
1Department of Biological Sciences, Purdue University

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Vi viser hvordan å dissekere og kultur chick E4 statoacoustic ganglion og E6 ryggmargen explants. Explants dyrkes i henhold til serum-frie forhold i 3D kollagen gels i 24 timer. Neurite respons er testet med vekstfaktor-supplert medium og med protein-belagt perler.

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Nevrovitenskap kylling disseksjon morphogen NT-3 neurite utvekst ryggmarg statoacoustic ganglion Wnt5a
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