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Journal / Neuroscience / Dissekering och kultur Chick Statoacoustic…
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Neuroscience

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JoVE Journal Neuroscience
Dissekering och kultur Chick Statoacoustic Ganglion och Spinal explants sladd i Collagen Gel för Neurite utväxt analyser
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Dissekering och kultur Chick Statoacoustic Ganglion och Spinal explants sladd i Collagen Gel för Neurite utväxt analyser

Article DOI: 10.3791/3600-v • 11:08 min • December 20th, 2011
December 20th, 2011 •
1, 1
1Department of Biological Sciences, Purdue University

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Vi visar hur du dissekera och kultur chick E4 statoacoustic ganglion och E6 spinal explants sladden. Explants odlas i serumfritt förhållanden i 3D kollagen geler i 24 timmar. Neurite lyhördhet testas med tillväxtfaktor-kompletterat medium och med protein-belagda pärlor.

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Neurovetenskap kyckling dissektion morphogen NT-3 neurite utväxt ryggmärg statoacoustic ganglion Wnt5a
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