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Immunology and Infection

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JoVE Journal Immunology and Infection
High-Throughput Nachweisverfahren für Influenza-Virus
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High-Throughput Nachweisverfahren für Influenza-Virus

Article DOI: 10.3791/3623-v • 10:05 min • February 4th, 2012
February 4th, 2012 •
1, 1, 2, 3, 4, 4, 1,5, 1,6
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

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Diese Methode beschreibt die Verwendung von Infrarot-Farbstoff basierende Imaging-System für den Nachweis von H1N1 in bronchioalveolar Lavage (BAL) Flüssigkeit von infizierten Mäuse mit einer hohen Empfindlichkeit. Diese Methodik in einem 96 durchgeführt werden kann - oder 384-Well-Platte, erfordert <10 ul Volumen von Testmaterial und hat das Potenzial für die gleichzeitige Screening von mehreren Erregern.

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Immunologie Influenza-Virus Virus-Titer Epithelzellen
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