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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput Metodo di rilevazione per il virus dell'influenza
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High-throughput Metodo di rilevazione per il virus dell'influenza

Article DOI: 10.3791/3623-v • 10:05 min • February 4th, 2012
February 4th, 2012 •
1, 1, 2, 3, 4, 4, 1,5, 1,6
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

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Questo metodo descrive l'utilizzo di Infrared sistema colorante di imaging based per il rilevamento di H1N1 in bronchioalveolar lavaggio (BAL) fluido di topi infettati ad una sensibilità elevata. Questa metodologia può essere eseguita in uno 96 - o 384-bene piastra, richiede <10 volume pl di materiale di prova e ha il potenziale per lo screening concomitante di agenti patogeni multipli.

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Immunology virus dell'influenza titolo Virus le cellule epiteliali
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