Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Isolation and Expansion of Human Glioblastoma…
JoVE Journal
Neuroscience
Author Produced

This content is Free Access.

JoVE Journal Neuroscience
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera
Journal (Neuroscience)
Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera
Immunization of Mouse with MOG35-55 to Induce Experimental Autoimmune Encephalomyelitis (Video) | JoVE
Journal (Neuroscience)
Immunization of Mouse with MOG35-55 to Induce Experimental Autoimmune Encephalomyelitis (Video) | JoVE
 

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

Article DOI: 10.3791/3633-v • 09:25 min • October 30th, 2011
October 30th, 2011 •
1,2, 1, 1, 1, 1, 1
1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences

Chapters

Summary
Automatic Translation

This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.

Tags

Isolation Expansion Human Glioblastoma Multiforme Tumor Cells Neurosphere Assay Stem-like Cells Breast Lung Colon Prostate Brain Tumor Initiating Cell Population(s) Tumor Formation Progression Recurrence Therapeutic Approaches Neurosphere Culture Method Surgically Resected Human GBM Tumor Tissue Chemical Digestion Mechanical Dissociation Single Cell Suspension NSA Culture Primary Neurospheres
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter