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JoVE Journal Bioengineering
Site-specifik bakteriekromosomet Engineering
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Site-specifik bakteriekromosomet Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Article DOI: 10.3791/3698-v • 08:21 min • March 16th, 2012
March 16th, 2012 •
1, 1, 1
1Biology, University of Waterloo

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En hurtig og effektiv metode til at integrere fremmed DNA af interesse i præfremstillede acceptor stammer, betegnet landingsplads stammer, er beskrevet. Fremgangsmåden tillader stedspecifik integration af et DNA-kassette i det konstruerede landingsplads locus af en given stamme, gennem konjugation og ekspression af det ΦC31 integrase.

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Bioengineering ΦC31 Integrase Rhizobiales Chromosome Engineering bakterielle genetik
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