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Fenotypische analyse en isolatie van Murine hematopoëtische stamcellen en Lineage-geëngageerde voorouders
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Fenotypische analyse en isolatie van Murine hematopoëtische stamcellen en Lineage-geëngageerde voorouders

Article DOI: 10.3791/3736-v • 12:03 min • July 8th, 2012
July 8th, 2012 •
1, 1, 1,2
1Institute for Research in Biomedicine, Bellinzona (Switzerland), 2Dipartimento di Biologia e Genetica per le Scienze Mediche, Universitá degli Studi di Milano

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Een methode om de verdeling van beenmerg hematopoietische stamcellen in flowcytometrie en efficiënt isoleren sterk gezuiverde hematopoietische stamcellen (HSC) analyse beschreven. De isolatie procedure is in hoofdzaak gebaseerd op magnetische verrijking van c-Kit + cellen en cel sortering tot HSCs voor cellulaire en moleculaire studies te zuiveren.

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Stem Cell Biology Moleculaire Biologie Geneeskunde Haematopoiese hematopoietische stamcellen hematopoietische voorlopercellen beenmerg flowcytometrie
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