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Journal / Immunology and Infection / Imagem do HIV-1 Synapse Envelope induzida…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Imagem do HIV-1 Synapse Envelope induzida virológica e Sinalização em bicamadas lipídicas sintéticas
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Imagem do HIV-1 Synapse Envelope induzida virológica e Sinalização em bicamadas lipídicas sintéticas

Article DOI: 10.3791/3757-v • 11:46 min • March 8th, 2012
March 8th, 2012 •
*1,2, *2,3, 1,2, 1,2, 2, 1,4
1Department of Pathology, New York University Langone School of Medicine, 2Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, 3Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 4Veteran Affairs New York Harbor Healthcare System
* These authors contributed equally

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Este artigo descreve um método para visualizar a formação de uma sinapse envelope de HIV-1 induzida por virológica em vidro suportados bicamadas planares por reflexão total interna de fluorescência microscopia (TIRF). O método pode também ser combinado com coloração de imunofluorescência para detectar a activação e redistribuição de moléculas de sinalização que ocorrem durante o HIV-1 a formação de sinapses envelope induzida virológica.

Tags

Imunologia microscopia TIRF bicamada planar envelope HIV sinapse virológica
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