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JoVE Journal
Biology

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从三维混合细胞球体共培养的乳腺上皮细胞的隔离
 
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从三维混合细胞球体共培养的乳腺上皮细胞的隔离

Article DOI: 10.3791/3760-v 07:27 min April 30th, 2012
April 30th, 2012

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Summary

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被形容为一个简单的方法,分析相关的上皮细胞组织成纤维细胞的影响。这种方法和三维组织培养相结​​合,可以方便从3D隔离后的细胞分析。该技术适用于不同恶性潜能的细胞,使系统的研究对肿瘤细胞的肿瘤相关基质的影响。

Tags

分子生物学,62期,肿瘤微环境,细胞外基质,三维,共培养,球体,混合细胞,细胞培养;
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