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Biology

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Profilering Thiol Redox Proteome Brug Isotope Tagging massespektrometri

Article DOI: 10.3791/3766-v • 12:08 min • March 24th, 2012
March 24th, 2012 •
1, 2, 2, 1,2,3,4
1Plant Molecular and Cellular Biology Program, University of Florida, 2Department of Biology, University of Florida, 3Interdisciplinary Center for Biotechnology Research, University of Florida, 4Genetics Institute, University of Florida

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Reaktive oxygenformer niveau hæves, når celler støder stressbetingelser. Her viser vi eksempel 3'-3 'diaminobenzidin farvning såvel som cysTMT mærkning og massespektrometri for at profilere redox proteom med

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Genetik , cystein-reaktiv tandem masse tag (cysTMT) LTQ-Orbitrap massespektrometri
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