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GC-baseret detektion af Aldononitrile Acetat Derivatiseret Glucosamin og Muramic Acid for Mikrobiel Rest bestemmelse i jord
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GC-baseret detektion af Aldononitrile Acetat Derivatiseret Glucosamin og Muramic Acid for Mikrobiel Rest bestemmelse i jord

Article DOI: 10.3791/3767-v • 11:06 min • May 19th, 2012
May 19th, 2012 •
1,2, 2, 2,3
1DOE-Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, 2Department of Soil Science, University of Wisconsin, Madison, 3Department of Soil and Water Science, University of Florida

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Beskriver vi en fremgangsmåde protokol for GC-analyse af aldonitrile acetat derivater af glucosamin og muramic syre ekstraheret fra jord. Til belysning af den kemiske mekanisme også præsenteres en strategi for at bekræfte strukturen af ​​derivatet og ion-fragmenterne dannet ved elektron ionisering.

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Molekylær Biologi Glucosamin muramic syre mikrobiel rester aldononitrile acetat derivatisering isotop inkorporering ion-struktur elektron ionisering GC MS
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