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Bioengineering
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Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish
 

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

Article DOI: 10.3791/3780-v 10:33 min September 30th, 2012
September 30th, 2012

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Summary

A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport. We demonstrate the use of this device and show examples of different types of data collected from different model systems.

Tags

Microfluidic Devices In Vivo Imaging C. Elegans Drosophila Zebrafish Soft Lithography Techniques Sub-cellular Imaging Laser Microsurgery Cellular Imaging Immobilization Techniques Suction Tapered Channels Deformable Membranes Anesthetic-free Immobilization PDMS Device Genetic Model Organisms
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