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Journal / Bioengineering / Spatio-Temporal Manipulation of Small GTPase…
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Bioengineering

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JoVE Journal Bioengineering
Spatio-Temporal Manipulation of Small GTPase aktivitet på subcellulære nivå og på Tidsskala sekunder i levende celler
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Spatio-Temporal Manipulation of Small GTPase aktivitet på subcellulære nivå og på Tidsskala sekunder i levende celler

Article DOI: 10.3791/3794-v • 10:28 min • March 9th, 2012
March 9th, 2012 •
1, 1, 1,2, 1,2, 2, 1,3, 1
1Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, 2Graduate School of Pharmaceutical Sciences, University of Tokyo, 3Biomedical Engineering, Johns Hopkins University

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En metode for spatio-temporal kontroll av små GTPase aktivitet av lys er beskrevet. Denne metoden er basert på rapamycin-indusert FKBP-FRB heterodimerization og foto-merd systemer. Optimalisering av lys-bestråling muliggjør spatio-timelig kontrollert aktivering av små GTPases på subcellulære nivå.

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Bioteknologi Small GTPase rapamycin bur sammensatte spatiotemporal kontroll heterodimerization FKBP FRB lys bestråling
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