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Real-time overvågning af ligand-receptor interaktioner med Fluorescence Resonance Energy Transfer
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Real-time overvågning af ligand-receptor interaktioner med Fluorescence Resonance Energy Transfer

Article DOI: 10.3791/3805-v • 12:23 min • August 20th, 2012
August 20th, 2012 •
1, 1, 1, 1
1Department of Chemistry and Biochemistry, Southern Illinois University

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Vi viser FRET mellem konjugeret polymer polydiacetylene (PDA) og fluorofor bundet til overfladen af ​​PDA liposomer til affølingen af ​​biomolekyler. PDA liposomer indeholdt også receptormolekyler på deres overflader for biomolekyler, der skal anvendes som prober. Ligand-receptor-interaktioner fører til ændringer i FRET effektivitet mellem fluoroforen og PDA som er grundlaget for følemekanisme.

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Biokemi molekylærbiologi kemi fysik Fluorescence Resonance Energy Transfer (FRET) Polydiacetylene (PDA) Biosensor Liposome Sansning
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