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Journal / Immunology and Infection / Ingegneria ed evoluzione del sintetico virus…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Ingegneria ed evoluzione del sintetico virus adeno-associato (AAV) Vettori Terapia genica attraverso la famiglia Shuffling DNA
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Ingegneria ed evoluzione del sintetico virus adeno-associato (AAV) Vettori Terapia genica attraverso la famiglia Shuffling DNA

Article DOI: 10.3791/3819-v • 21:56 min • April 2nd, 2012
April 2nd, 2012 •
*1, *1, 2, 1, 1, 1, 1
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University
* These authors contributed equally

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Dimostriamo la tecnica di base per progettare molecolare ed evolvere sintetici virale adeno-associati (AAV) vettori di terapia genica attraverso la famiglia shuffling DNA. Inoltre, fornire linee guida generali ed esempi rappresentativi per la selezione e l'analisi delle singole capsidi chimerici con proprietà migliorate sulle cellule bersaglio in coltura o nei topi.

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Immunology virus adeno-associato AAV terapia genica biologia sintetica vettore virale evoluzione molecolare DNA shuffling
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