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Journal / Immunology and Infection / Engineering och evolution av syntetisk…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Engineering och evolution av syntetisk Adeno-associerat virus (AAV) Gene Therapy Vektorer via DNA Familj Skyffling
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
Journal (Immunology and Infection)
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
Journal (Immunology and Infection)
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
 
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Engineering och evolution av syntetisk Adeno-associerat virus (AAV) Gene Therapy Vektorer via DNA Familj Skyffling

Article DOI: 10.3791/3819-v • 21:56 min • April 2nd, 2012
April 2nd, 2012 •
*1, *1, 2, 1, 1, 1, 1
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University
* These authors contributed equally

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Vi visar den grundläggande tekniken för att molekylärt konstruera och utveckla syntetiska Adeno-associerade virus (AAV) vektorer för genterapi via DNA-familjen skyffling. Dessutom ger vi allmänna riktlinjer och representativa exempel för urval och analys av enskilda chimärakapsider med förbättrade egenskaper på målceller i kultur eller möss.

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Immunologi utgåva 62 adeno-associerat virus AAV genterapi syntetisk biologi viral vektor molekylär evolution DNA-skyffling
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