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Journal / Immunology and Infection / Een primaire Neuron Cultuurstelsel voor de Studie …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Een primaire Neuron Cultuurstelsel voor de Studie van Herpes Simplex Virus latentie en reactivatie
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Een primaire Neuron Cultuurstelsel voor de Studie van Herpes Simplex Virus latentie en reactivatie

Article DOI: 10.3791/3823-v • 12:22 min • April 2nd, 2012
April 2nd, 2012 •
1, 1, 2, 3, 2,4,5,6,7, 1, 1
1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

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Het protocol beschrijft een efficiënt en reproduceerbaar modelsysteem om te studeren herpes simplex virus type 1 (HSV-1) latentie en reactivatie. De test maakt gebruik van homogene sympathiek neuron culturen en maakt het mogelijk om moleculaire dissectie van virus-neuron interacties met behulp van een scala aan hulpmiddelen met inbegrip van RNA-interferentie en expressie van recombinante eiwitten.

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Immunologie neuron celkweek Herpes Simplex Virus (HSV) moleculaire biologie virologie
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