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Immunology and Infection

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JoVE Journal Immunology and Infection
Un sistema de neuronas cultivo primario para el estudio de la latencia del virus del herpes simple y Reactivación
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Un sistema de neuronas cultivo primario para el estudio de la latencia del virus del herpes simple y Reactivación

Article DOI: 10.3791/3823-v • 12:22 min • April 2nd, 2012
April 2nd, 2012 •
1, 1, 2, 3, 2,4,5,6,7, 1, 1
1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

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El protocolo se describe un sistema modelo eficiente y reproducible para estudiar virus herpes simplex tipo 1 (VHS-1) latencia y reactivación. El ensayo emplea cultivos de neuronas simpáticas homogéneos y permite la disección molecular de las interacciones neurona-virus utilizando una variedad de herramientas incluyendo la interferencia de ARN y la expresión de proteínas recombinantes.

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Inmunología Número 62 la cultura de neuronas de células virus del herpes simple (VHS) la biología molecular virología
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