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JoVE Journal Bioengineering
Dyrkning af humane neurale stamceller i en 3-dimensionel selvsamlende peptid Hydrogel
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Dyrkning af humane neurale stamceller i en 3-dimensionel selvsamlende peptid Hydrogel

Article DOI: 10.3791/3830-v • 11:02 min • January 11th, 2012
January 11th, 2012 •
1, 1, 1
1Albrecht-Kossel-Institute for Neuroregeneration, University of Rostock

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Her beskriver vi brug af en selvsamlende 3-dimensionelle stillads til kultur menneskelige neurale stamceller. Vi præsenterer en protokol for at frigøre celler fra stilladser, der skal analyseres efterfølgende fx ved flowcytometri. Denne protokol kan tilpasses til andre celletyper at udføre detaljerede mekanisk undersøgelser.

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Bioteknik PuraMatrix RADA16 3D-stillads ReNcell VM menneskelige neurale stamceller kvantificering
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