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MicroRNA Påvisning i prostata tumorer ved kvantitativ real-time PCR (qPCR)
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MicroRNA Påvisning i prostata tumorer ved kvantitativ real-time PCR (qPCR)

Article DOI: 10.3791/3874-v • 08:30 min • May 16th, 2012
May 16th, 2012 •
1, 2, 3, 1, 1,3,4
1Department of Laboratory Medicine & Pathobiology, University of Toronto, 2Division of Urology, Sunnybrook Health Sciences Centre, Toronto, Canada, 3Department of Anatomic Pathology, Sunnybrook Health Sciences Centre, Toronto, Canada, 4Biological Sciences, Sunnybrook Research Institute

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Kvantitativ realtids polymerasekædereaktion (qPCR) er en hurtig og følsom metode til at undersøge ekspressionsniveauerne af forskellige microRNA (miRNA) molekyler i tumorprøver. Anvendelse af denne fremgangsmåde ekspression af hundreder af forskellige miRNA molekyler kan forstærkes, kvantificeres, og analyseres fra det samme cDNA-skabelon.

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Cancer Biology medicin kræft primer analyse prostata microRNA tumor qPCR
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