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MicroRNA-Detektion in Prostatatumoren durch quantitative real-time PCR (qPCR)

Article DOI: 10.3791/3874-v • 08:30 min • May 16th, 2012
May 16th, 2012 •
1, 2, 3, 1, 1,3,4
1Department of Laboratory Medicine & Pathobiology, University of Toronto, 2Division of Urology, Sunnybrook Health Sciences Centre, Toronto, Canada, 3Department of Anatomic Pathology, Sunnybrook Health Sciences Centre, Toronto, Canada, 4Biological Sciences, Sunnybrook Research Institute

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Quantitative Real Time Polymerase-Kettenreaktion (qPCR) ist eine schnelle und empfindliche Methode, um die Expression verschiedener microRNA (miRNA) Moleküle in Tumorproben zu untersuchen. Unter Verwendung dieses Verfahrens Expression von Hunderten von verschiedenen miRNA-Moleküle können verstärkt, quantifiziert werden, und analysiert die gleiche cDNA-Matrize.

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Cancer Biology Ausgabe 63 Medizin Krebs Primer-Assay Prostata- microRNA Tumor qPCR
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