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Journal / Bioengineering / Mikropipett Aspiration av substrat-anslutna…
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Bioengineering

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JoVE Journal Bioengineering
Mikropipett Aspiration av substrat-anslutna celler att uppskatta Cell Styvhet
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Mikropipett Aspiration av substrat-anslutna celler att uppskatta Cell Styvhet

Article DOI: 10.3791/3886-v • 10:31 min • September 27th, 2012
September 27th, 2012 •
1, 1, 2, 1
1Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois, 2Institute for Medicine and Engineering, University of Pennsylvania

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Här beskriver vi en snabb och enkel metod för att mäta cell styvhet. Den allmänna principen i denna strategi är att mäta membran deformation som svar på väldefinierade undertryck appliceras via en mikropipett till cellytan. Denna metod ger ett kraftfullt verktyg för att studera biomekaniska egenskaper hos substrat-anslutna celler.

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Bioteknik biofysik medicinsk teknik medicin Cellulär biologi cell stelhet biomekanik microaspiration cellmembran cytoskelettet
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