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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

핫 수성 - 페놀 추출에 의한 그람 음성 박테리아에서 정화와 Lipopolysaccharide의 시각화

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Journal (Immunology and Infection)

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

Journal (Immunology and Infection)

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

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핫 수성 - 페놀 추출에 의한 그람 음성 박테리아에서 정화와 Lipopolysaccharide의 시각화

Article DOI: 10.3791/3916-v • 05:32 min • May 28th, 2012

May 28th, 2012 •

Michael R. Davis, Jr.¹, Joanna B. Goldberg¹

¹Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

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우리는 그람 음성 세균의 lipopolysaccharide을 (LPS) 정화를위한 수정된 뜨거운 수성 - 페놀 추출 방법을 설명합니다. 일단 압축을 푼, LPS는 연속적으로 SDS-PAGE에 의해 분석 및 직접 염색법이나 서양 immunoblot에 의해 시각하실 수 있습니다.

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면역학 이슈 63 그람 음성 LPS 추출 다당류의 염색법 서양 immunoblot

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