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Journal / Immunology and Infection / Rensing og Visualisering av lipopolysakkarid fra…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Rensing og Visualisering av lipopolysakkarid fra Gram-negative bakterier ved Hot Vandig-fenol Extraction
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Rensing og Visualisering av lipopolysakkarid fra Gram-negative bakterier ved Hot Vandig-fenol Extraction

Article DOI: 10.3791/3916-v • 05:32 min • May 28th, 2012
May 28th, 2012 •
1, 1
1Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

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Vi beskriver en modifisert hot vandig-fenol utvinning metode for rensing av lipopolysakkarid (LPS) fra Gram-negative bakterier. Når utvinnes, kan LPS bli senere analyseres av SDS-PAGE og visualisert ved direkte farging eller vestlig immunoblot.

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Immunologi Gram-negative LPS utvinning polysakkarid farging Vest immunoblot
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