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Isolamento e cultura primária de células hepáticas de ratos
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Isolamento e cultura primária de células hepáticas de ratos

Article DOI: 10.3791/3917-v • 07:06 min • June 29th, 2012
June 29th, 2012 •
1, 2, 3, 1
1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 2American University in Washington, D.C., 3Department of Internal Medicine, Saint Louis University School of Medicine

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Hepatócitos primários proporcionar uma ferramenta valiosa para avaliar as funções bioquímicas, moleculares, e metabólicas em um sistema fisiologicamente relevante experimental. Nós descrevemos um protocolo confiável para rato em perfusão de fígado in situ, que sempre gera hepatócitos viáveis ​​até to1.0 × 10

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Biologia Celular fisiologia medicina cultura de células primárias células hepáticas no rato hepatócitos
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