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Hurtig Fibroblast fjernelse fra High Density humane embryonale stamceller Cultures
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Hurtig Fibroblast fjernelse fra High Density humane embryonale stamceller Cultures

Article DOI: 10.3791/3951-v • 07:12 min • October 28th, 2012
October 28th, 2012 •
1, 1
1Department of Engineering, University of California, Merced

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Trods den stadige bestræbelser på at overgangen kulturer til feeder-fri betingelser, forbliver afledning og dyrkning af humane embryonale stamceller (hESC) i høj grad afhængig af co-kulturer med muse embryonale foderautomater (MEF). Her viser vi en ny metode til hurtigt at fjerne foderautomater fra hESC kulturer før eksperimenteren.

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Cellular Biology humane embryonale stamceller Cell Culture Cell Isolation Oct Cell Purification MEF Removal SSEA-4
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