Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

* These authors contributed equally
Published 6/22/2012
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Bioengineering

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Summary

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Myers, D. R., Sakurai, Y., Tran, R., Ahn, B., Hardy, E. T., Mannino, R., et al. Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases. J. Vis. Exp. (64), e3958, doi:10.3791/3958 (2012).

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Abstract

Materials

Name Company Catalog Number Comments
blunt point needle OK International 920050-TE Precision TE needle 20 Gauge x 1/2", pink
dextran Sigma-Aldrich 31392
Fibronectin Sigma-Aldrich F0895
Hole puncher (pin vise) Technical Innovations
Human umbilical cord endothelial cells (HUVECs) Lonza CC-2519
Plasma cleaner Plasma PDC-326
Polydimethylsiloxane (PDMS) Fisher Scientific NC9285739 Sylgard 184 Silicone Elastomer KIT
Sigmacote Sigma-Aldrich SL2
SU-8 2025 Microchem Y111069
SU-8 Developer Microchem Y020100
Syringe pump Harvard Apparatus 70-3008 PHD-ULTRA
tubing(larger) Cole-Parmer Instrument Company 06418-02 Tygonreg microbore tubing, 0.020" ID x 0.060" OD
tubing(smaller) Cole-Parmer Instrument Company 06417-11 PTFE microbore tubing, 0.012" ID x 0.030" OD

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References

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  4. Higgins, J. M., Eddington, D. T., Bhatia, S. N., Mahadevan, L. Sickle cell vasoocclusion and rescue in a microfluidic device. Proceedings of the National Academy of Sciences. 104, 20496-20500 (2007).
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  14. Tsai, M. In vitro modeling of the microvascular occlusion and thrombosis that occur in hematologic diseases using microfluidic technology. The Journal of Clinical Investigation. Forthcoming (2011).
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Comments

1 Comment

  1. Hello

    This is a very interesting and clever way to produce vascularized microfluidic device. Congratulations for the nice work. I have one question regarding this:

    In the video, it is mentioned that the sickle RBCs were deoxygenated: so how the de-oxygenation was achieved and how was the de-oxygenation maintained during the flow as PDMS is known to be Oxygen-permeable?

    Thank you

    Reply
    Posted by: ANUPAM A.
    August 16, 2015 - 7:04 PM

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