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Bioengineering

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JoVE Journal Bioengineering
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Article DOI: 10.3791/3977-v • 13:48 min • May 29th, 2012
May 29th, 2012 •
1,2, 2, 2,3, 2,4, 1,2
1Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST), Eberhard Karls University, Tübingen, 2Department of Cell and Tissue Engineering, Fraunhofer Institute of Interfacial Engineering and Biotechnology (IGB) Stuttgart, Germany, 3Department for Medical Interfacial Engineering (IGVT), University of Stuttgart, Germany, 4Institute of Tissue Engineering and Regenerative Medicine, Julius-Maximillians University, Würzburg, Germany

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Summary
Automatic Translation

Raman spectroscopy is a suitable technique for the non-contact, label-free analysis of living cells, tissue-engineered constructs and native tissues. Source-specific spectral fingerprints can be generated and analyzed using multivariate analysis.

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Raman Spectroscopy Non-contact Monitoring Label-free Monitoring Cells Extracellular Matrix Biomedical Research Non-destructive Laser-based Technology Spectral Pattern Chemical Vibration Raman Band Peak Intensities Multivariate Analysis Principal Component Analysis (PCA) Living Cells Native Tissues Glass Bottom Dishes Cell Culture Conditions Cell Nucleus ECM Proteins
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