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Nano-FEM: Protein Localization brug af foto-aktiverede Localization mikroskopi og elektronmikroskopi

Article DOI: 10.3791/3995-v • 13:13 min • December 3rd, 2012
December 3rd, 2012 •
1, 1, 1, 1, 1, 1
1Department of Biology, Howard Hughes Medical Institute, University of Utah

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Vi beskriver en fremgangsmåde til at lokalisere fluorescerende mærkede proteiner i elektronmikrofotografier. Fluorescens først lokaliseret ved brug af foto-aktiveret lokalisering mikroskopi på ultratynde sektioner. Disse billeder bliver derefter tilpasset elektronmikrografer af den samme sektion.

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Molekylær Biologi Cellular Biology Genetics Proteomics Proteins Protein lokalisering super-opløsning fluorescensmikroskopi fluorescens elektronmikroskopi nano-five EM SEM elektronmikrografi billedbehandling
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