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Einsatz eines Roboters für High-Throughput-Kristallisation von Membranproteinen in Lipidische Mesophasen
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Einsatz eines Roboters für High-Throughput-Kristallisation von Membranproteinen in Lipidische Mesophasen

Article DOI: 10.3791/4000-v • 20:22 min • September 1st, 2012
September 1st, 2012 •
1, 1, 1, 1
1Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin

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Hierin wird eine Roboter-Ansatz zur Hochdurchsatz-Kristallisation von Membranproteinen in lipidische Mesophasen zur Verwendung in Strukturbestimmung mittels makromolekularen Röntgenkristallographie beschrieben. Drei Roboter in der Lage Umgang mit dem viskosen und klebrigen Protein-beladenen Mesophase integraler Bestandteil der Methode eingeführt werden.

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Zellbiologie Automatisierungs- Kristallisations- Glas-Sandwich-Platten mit hohem Durchsatz, Lipidischen kubischen Phase Lipid-Mesophasen makromolekulare Röntgenkristallographie Membranprotein Rezeptor-Roboter
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