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Journal / Biology / Isolierung von Ribosom gebunden Nascent…
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Isolierung von Ribosom gebunden Nascent Polypeptide
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Isolierung von Ribosom gebunden Nascent Polypeptide

Article DOI: 10.3791/4026-v • 10:15 min • July 6th, 2012
July 6th, 2012 •
1, 1
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

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Eine Technik zur translationalen Pause Sites auf mRNA zu identifizieren beschrieben wird. Dieses Verfahren beruht auf der Trennung entstehenden Polypeptide ansammeln auf Ribosomen während der in vitro Translation einer Ziel-mRNA, durch die Größe Analyse der naszierenden Ketten mit Hilfe eines denaturierenden Gelelektrophorese gefolgt basiert.

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Genetik Ausgabe 65 Molekularbiologie Ribosom entstehenden Polypeptid Co-translationale Proteinfaltung gleichbedeutend Codonverwendung Genregulation
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