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Utilizzando Sequence SECM arresto come uno strumento per isolare ribosoma Polipeptidi Bound
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Utilizzando Sequence SECM arresto come uno strumento per isolare ribosoma Polipeptidi Bound

Article DOI: 10.3791/4027-v • 09:42 min • June 19th, 2012
June 19th, 2012 •
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1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

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Descriviamo qui una tecnica che viene ora utilizzato di routine per isolare complessi ribosoma stabilmente legati a catena nascente (RNC). Questa tecnica sfrutta la scoperta che un 17 aminoacidi lungo "sequenza di arresto" SECM può fermare l'allungamento traduzione in un procariota (

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Biologia molecolare numero 64 ribosoma polipeptidi nascenti co-traslazionale folding delle proteine arresto traslazionale,
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