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Brug SecM Arrest Sequence som et redskab til at isolere ribo bundne polypeptider
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Brug SecM Arrest Sequence som et redskab til at isolere ribo bundne polypeptider

Article DOI: 10.3791/4027-v • 09:42 min • June 19th, 2012
June 19th, 2012 •
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1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

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Vi beskriver her en teknik, der nu rutinemæssigt anvendes til at isolere stabilt bundet ribosom vordende kæde-komplekser (RNC'er). Denne teknik udnytter den erkendelse, at en 17 aminosyre langt SecM "arrest sekvens" kan standse translation forlængelse i en prokaryot (

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Molekylær Biologi ribosomet spirende polypeptider co-translationel proteinfoldning translationel anholdelse,
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