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Bruke SecM Arrest sekvens som et verktøy for å isolere ribosom Innbundne polypeptider
Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
Journal (Biology)
Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
Journal (Biology)
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
 
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Bruke SecM Arrest sekvens som et verktøy for å isolere ribosom Innbundne polypeptider

Article DOI: 10.3791/4027-v • 09:42 min • June 19th, 2012
June 19th, 2012 •
1, 1
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

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Vi beskriver her en teknikk som nå rutinemessig brukt til å isolere stabilt innbundne ribosomet begynnende kjeden komplekser (RNCs). Denne teknikken utnytter oppdagelsen av at en 17 aminosyre lang SecM "arrest sekvens" kan stanse oversettelse tøyelighet i en prokaryot (

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Molecular Biology ribosomet begynnende polypeptider co-translasjonell protein folding translasjonsforskning arrest,
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