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Immunology and Infection

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JoVE Journal Immunology and Infection
Affinity Rensing av influensavirus ribonucleoprotein Komplekser fra Chromatin av infiserte celler
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Affinity Rensing av influensavirus ribonucleoprotein Komplekser fra Chromatin av infiserte celler

Article DOI: 10.3791/4028-v • 11:20 min • June 3rd, 2012
June 3rd, 2012 •
1, 1
1Department of Virology, Universitätsklinikum Freiburg

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Influensavirus kopiere deres RNA genomet i forbindelse med host-celle kromatin. Her presenterer vi en metode for å rense intakte viral ribonucleoprotein komplekser fra kromatin av infiserte celler. Renset virale komplekser kan analyseres av både Western blot og primer forlengelse av protein og RNA innhold, henholdsvis.

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Virologi Molecular Biology Influensa A virus slektskap rensing subcellulære fraksjonering kromatin vRNP komplekser polymerase
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