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JoVE Journal Neuroscience
Produktion av Lentiviral vektorer för att omvandla Celler från det centrala nervsystemet
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Produktion av Lentiviral vektorer för att omvandla Celler från det centrala nervsystemet

Article DOI: 10.3791/4031-v • 08:46 min • May 24th, 2012
May 24th, 2012 •
1, 1, 1, 1
1Department of Neurology and Hope Center for Neurological Disorders, Washington University School of Medicine

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I detta protokoll beskriver vi produktion, rening och titrering av lentivirala vektorer. Vi erbjuder ett exempel på lentivirusvektor-medierad gen leverans i primära odlade nervceller och astrocyter. Våra metoder kan också tillämpas på andra celltyper

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Neuroscience utgåva 63 cellodling transduktion lentiviral vektor neuron astrocyt promotor CNS- genetik
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