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JoVE Journal Neuroscience
De productie van lentivirale vectoren voor transduceren Cellen van het centrale zenuwstelsel
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De productie van lentivirale vectoren voor transduceren Cellen van het centrale zenuwstelsel

Article DOI: 10.3791/4031-v • 08:46 min • May 24th, 2012
May 24th, 2012 •
1, 1, 1, 1
1Department of Neurology and Hope Center for Neurological Disorders, Washington University School of Medicine

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In dit protocol beschrijven we de productie, zuivering en titratie van lentivirale vectoren. Wij bieden een voorbeeld van lentivirale vector-gemedieerde gen-levering in de eerste gekweekte neuronen en astrocyten. Onze methoden kunnen ook van toepassing op andere celtypen

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Neuroscience Cell cultuur transductie lentivirale vector neuron astrocyten promotor CNS genetica
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