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A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development

Article DOI: 10.3791/4035-v • 11:17 min • June 7th, 2012
June 7th, 2012 •
1, 1, 1
1Department of Microbiology and Immunology, Loyola University Medical Center

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We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.

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Semi-quantitative Approach Assess Biofilm Formation Wrinkled Colony Development Bacteria Extracellular Matrix Physiology Resistance To Antibiotics Environmental Stresses Host-microbe Interactions Planktonic Cells Complex Communities Laboratory Study Biofilm Phenotypes Wrinkled Or Rugose Bacterial Colonies Solid Agar Media Indicator Of Biofilm Formation Gram-positive Bacteria Gram-negative Bacteria Bacillus Subtilis Vibrio Cholerae Vibrio Parahaemolyticus Pseudomonas Aeruginosa Vibrio Fischeri
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