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Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
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Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice

Article DOI: 10.3791/404-v • 10:44 min • October 31st, 2007
October 31st, 2007 •
1, 1, 1
1Diabetes Center, University of California, San Francisco - UCSF

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Our protocol was developed to cleanly and easily deliver islets or cells under the kidney capsule of mice. Cells are concentrated into pellets in the final tubing used for transplanting the cells under the kidney capsule. The ease of this technique reduces stress to the cells and the mouse.

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Transplantation Pancreatic Islets Kidney Capsule Diabetic Mice Protocol Cells Delivery Tubing PE50 Concentration Pellets Speed Ease Stress Reduction Loading Microcentrifuge Tube Pipetteman Pipette Tip PE50 Tubing Attachment Silicone Adapter Tubing Settling Dialing Kink 15 ML Conical Centrifuging Anesthesia Shaving Cleaning Incision Kidney Exposure
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