Isolierung und Charakterisierung von dendritischen Zellen und Makrophagen aus dem Mausdarm

Published 5/21/2012
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Immunology and Infection

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Summary

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Geem, D., Medina-Contreras, O., Kim, W., Huang, C. S., Denning, T. L. Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. J. Vis. Exp. (63), e4040, doi:10.3791/4040 (2012).

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Abstract

Materials

Name Company Catalog Number Comments
1X PBS, Ca2+- and Mg2+-free
Hank's balanced salt solution (HBSS) with phenol red Fisher Scientific SH3001603
Sodium bicarbonate Sigma-Aldrich S6014
1M HEPES in 0.85% NaCl Lonza Inc. 17-737E
Fetal bovine serum (FBS) Atlanta Biologicals S11150H Heat-inactivated
0.5M EDTA (pH 8.0) Cellgro 46-034-CI
Collagenase type VIII Sigma-Aldrich C2139
DNase I Roche Group 14785000 Stock solution: 100mg/ml
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit for 405 nm excitation Invitrogen L34957 Use at 1:1000
CD45-PerCP mAb (30F11) BD Biosciences 557235 Use at 1:100
CD103-PE mAb (M290) BD Biosciences 557495 Use at 1:100
FcγRIII/II mAb (2.4G2) BD Biosciences 553141 Use at 1:200
CD11c-APC mAb (N418) eBioscience 17-0114-82 Use at 1:100
MHC-II (I-Ab)-Alexa Fluor 700 mAb eBioscience 56-5321-82 Use at 1:100
CD11b-eFluor 450 mAb (M1/70) eBioscience 48-0112-82 Use at 1:200
F4/80-PE-Cy7 mAb (BM8) eBioscience 25-4801-82
CD11b microbeads Miltenyi Biotec 130-049-601
CD11c microbeads Miltenyi Biotec 130-052-001
50 mL conical tubes BD Biosciences 352098
Single mesh wire strainer Chefmate
Small weigh boat Fisher Scientific 08-732-116
100 μm cell strainer BD Biosciences 352360
40 μm cell strainer BD Biosciences 352340
5 mL polystyrene round-bottom tubes BD Biosciences 352235 Use at 1:100
MaxQ 4450 benchtop orbital shaker Thermo Fisher Scientific, Inc.
LS MACS column Miltenyi Biotec 130-042-401
LSR II BD Biosciences
FACSAria II BD Biosciences

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References

  1. Maloy, K. J., Powrie, F. Intestinal homeostasis and its breakdown in inflammatory bowel disease. Nature. 474, 298-306 (2011).
  2. Nagler-Anderson, C., Terhoust, C., Bhan, A. K., Podolsky, D. K. Mucosal antigen presentation and the control of tolerance and immunity. Trends Immunol. 22, 120-122 (2001).
  3. Abraham, C., Medzhitov, R. Interactions between the host innate immune system and microbes in inflammatory bowel disease. Gastroenterology. 140, 1729-1737 (2011).
  4. Macdonald, T. T., Monteleone, I., Fantini, M. C., Monteleone, G. Regula tine. Gastroenterology. 140, 1768-1775 (2011).
  5. Rescigno, M. Intestinal dendritic cells. Adv. Immunol. 107, 109-138 (2010).
  6. Platt, A. M., Bain, C. C., Bordon, Y., Sester, D. P., Mowat, A. M. An independent subset of TLR expressing CCR2-dependent macrophages promotes colonic inflammation. J. Immunol. 184, 6843-6854 (2010).
  7. Coombes, J. L., Powrie, F. Dendritic cells in intestinal immune regulation. Nat. Rev. Immunol. 8, 435-446 (2008).
  8. Kelsall, B. Recent progress in understanding the phenotype and function of intestinal dendritic cells and macrophages. Mucosal Immunol. 1, 460-469 (2008).
  9. Pulendran, B., Tang, H., Denning, T. L. Division of labor, plasticity, and crosstalk between dendritic cell subsets. Curr. Opin. Immunol. 20, 61-67 (2008).
  10. Denning, T. L., Wang, Y. C., Patel, S. R., Williams, I. R., Pulendran, B. Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses. Nat. Immunol. 8, 1086-1094 (2007).
  11. Niess, J. H. CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance. Science. 307, 254-258 (2005).
  12. Milling, S. W., Cousins, L., MacPherson, G. G. How do DCs interact with intestinal antigens. Trends Immunol. 26, 349-352 (2005).
  13. Bilsborough, J., Viney, J. L. Gastrointestinal dendritic cells play a role in immunity, tolerance, and disease. Gastroenterology. 127, 300-309 (2004).
  14. Stagg, A. J., Hart, A. L., Knight, S. C., Kamm, M. A. The dendritic cell: its role in intestinal inflammation and relationship with gut bacteria. Gut. 52, 1522-1529 (2003).
  15. Medina-Contreras, O. CX3CR1 regulates intestinal macrophage homeostasis, bacterial translocation, and colitogenic Th17 responses in mice. J. Clin. Invest. 121, 4787-4795 (2011).
  16. Denning, T. L. Functional Specializations of Intestinal Dendritic Cell and Macrophage Subsets That Control Th17 and Regulatory T Cell Responses Are Dependent on the T Cell/APC Ratio, Source of Mouse Strain, and Regional Localization. J. Immunol. 187-733 (2011).
  17. Kim, Y. G. The Nod2 sensor promotes intestinal pathogen eradication via the chemokine CCL2-dependent recruitment of inflammatory monocytes. Immunity. 34, 769-780 (2011).
  18. Schulz, O. Intestinal CD103+, but not CX3CR1+, antigen sampling cells migrate in lymph and serve classical dendritic cell functions. J. Exp. Med. 206, 3101-3114 (2009).
  19. Jaensson, E. Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J. Exp. Med. 205, 2139-2149 (2008).
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Comments

5 Comments

  1. Hi,

    This is probably your most frequently asked questions. I have the correct Collagenase and I add 30 mg to the ²0 mL prewarmed HBSS with ² mM EDTA. Despite 1 hour at 37 at ²00 the intestine is very stubborn and refuses to digest. I mince it just as described. Any thoughts?? Should I work on increasing the collagenase concentration?

    Many thanks

    Reply
    Posted by: Shehzad S.
    June 13, 2012 - 8:53 AM
  2. Hello there,

    Thank you for your interest in the protocol. Your question seems very perplexing as we have never experienced difficulties with tissue digestion durations greater than 15 minutes, and you seem to be having difficulties after 1 hour. We do not use the ² mM EDTA during tissue digestion and the recommendations I can make are: do not digest the tissue for 1 hour, ensure that you are using an orbital shaker with the 50 ml conical tubes lying horizontally, mince the tissue more finely, and vortex for 50 ml conical tubes after digestion for about 15 to ²0 seconds so that any remaining intestinal tissue is dissociated and digested (you may get some strands of stromal tissue remaining at the end, which is normal). Hope this helps! Please cite our work if you utilize our protocol! Thank you.

    Reply
    Posted by: Duke G.
    June 22, 2012 - 11:16 AM
  3. who can i have a showed vidio play. i want to waching move

    Reply
    Posted by: lee j.
    October 18, 2012 - 9:17 AM
  4. Hi,

    I am using type IV collagenase. I am wondering what U/mg you are using the the type VIII collagenase at?

    Cheers!

    Reply
    Posted by: Mark D.
    November 20, 2012 - 3:17 PM
  5. also,

    I was wondering what is the total cell count you get from 1 small intestine and what cell count you get of cd11c cells per intestine.

    Thanks

    Reply
    Posted by: Mark D.
    November 21, 2012 - 1:27 PM

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