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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering og karakterisering af dendritiske celler og makrofager fra mus Tarmen
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Isolering og karakterisering af dendritiske celler og makrofager fra mus Tarmen

Article DOI: 10.3791/4040-v • 09:25 min • May 21st, 2012
May 21st, 2012 •
1, 1, 2, 1, 1,2
1Department of Pediatrics, Emory University, 2Department of Pathology & Laboratory Medicine, Emory University

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Her har vi detalje en metode til hurtig isolering af muse intestinale dendritiske celler (DCS) og makrofager. Fænotypisk karakterisering af intestinale DC'er og makrofager udføres under anvendelse af flerfarvede flowcytometrisk analyse under magnetisk perle tilsætning efterfulgt af cellesortering anvendes til opnåelse af særdeles rene populationer til funktionelle undersøgelser.

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Immunologi tarm immunologi APC'er dendritiske celler makrofager cellekultur
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