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JoVE Journal Immunology and Infection
Isolatie en karakterisering van dendritische cellen en macrofagen van de muis Darm
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Isolatie en karakterisering van dendritische cellen en macrofagen van de muis Darm

Article DOI: 10.3791/4040-v • 09:25 min • May 21st, 2012
May 21st, 2012 •
1, 1, 2, 1, 1,2
1Department of Pediatrics, Emory University, 2Department of Pathology & Laboratory Medicine, Emory University

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Hier hebben we details van een methodologie voor de snelle isolatie van de muis intestinale dendritische cellen (DC's) en macrofagen. Fenotypische karakterisering van intestinale DCs en macrofagen wordt uitgevoerd met behulp van multi-kleuren flowcytometrische analyse, terwijl magnetische kraal verrijking gevolgd door celsortering wordt gebruikt om zeer zuivere populaties voor functionele studies opleveren.

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Immunologie darm immunologie APC's dendritische cellen macrofagen celkweek
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