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Utarbeidelse av Akutt subventricular sone Slices for kalsium Imaging
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Utarbeidelse av Akutt subventricular sone Slices for kalsium Imaging

Article DOI: 10.3791/4071-v • 09:10 min • September 19th, 2012
September 19th, 2012 •
1, 1, 1, 1
1Department of Neurosurgery and Cellular & Molecular Physiology, Yale University School of Medicine

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En metode for å laste subventricular sone (SVZ) celler med kalsium indikator fargestoffer for opptak kalsium aktivitet er beskrevet. Postnatal SVZ inneholder tett pakket celler, inkludert nevrale stamceller og neuroblasts. Snarere enn å bruke bad lasting injisert vi fargestoff ved trykk inne i vevet lar bedre fargestoff diffusjon.

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Nevrovitenskap Molecular Biology anatomi fysiologi subventricular sone voksen neurogenesis gap veikryss kalsium bildebehandling nevrale stamceller
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