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JoVE Journal Neuroscience
Fremstilling af akutte Subventricular Zone Slices for Calcium Imaging
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Fremstilling af akutte Subventricular Zone Slices for Calcium Imaging

Article DOI: 10.3791/4071-v • 09:10 min • September 19th, 2012
September 19th, 2012 •
1, 1, 1, 1
1Department of Neurosurgery and Cellular & Molecular Physiology, Yale University School of Medicine

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En fremgangsmåde til at indlæse subventricular zone (SVZ) celler med calcium indikatorfarvestoffer til optagelse calcium aktivitet er beskrevet. Den postnatale SVZ indeholder tætpakkede celler, herunder neurale progenitorceller og neuroblaster. Snarere end at bruge bad loading vi injicerede farvestoffet ved tryk inde i vævet giver bedre farve diffusion.

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Neuroscience Molekylærbiologi anatomi fysiologi subventricular zone voksen neurogenese gap junction calcium billedbehandling neurale stamceller
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